Marker-free cell discrimination by holographic optical tweezers

F. Schaal; M. Warber; S. Zwick; H. van der Kuip; T. Haist; W. Osten

doi:10.2971/jeos.2009.09028

Journal of the European Optical Society - Rapid publications, Vol 4 (2009)

Marker-free cell discrimination by holographic optical tweezers

F. Schaal, M. Warber, S. Zwick, H. van der Kuip, T. Haist, W. Osten

Abstract

We introduce a method for marker-free cell discrimination based on optical tweezers. Cancerous, non-cancerous, and drug-treated cells could be distinguished by measuring the trapping forces using holographic optical tweezers. We present trapping force measurements on different cell lines: normal pre-B lymphocyte cells (BaF3; "normal cells"), their Bcr-Abl transformed counterparts (BaF3-p185; "cancer cells") as a model for chronic myeloid leukaemia (CML) and Imatinib treated BaF3-p185 cells. The results are compared with reference measurements obtained by a commercial flow cytometry system.

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