Journal of the European Optical Society - Rapid publications, Vol 7 (2012)

An integrated Young interferometer based on UV-imprinted polymer waveguides for label-free biosensing applications

M. Wang, J. Hiltunen, C. Liedert, L. Hakalahti, R. Myllylä

Abstract


We demonstrate a polymer rib waveguide Young interferometer sensor fabricated by UV-imprinting. An inverted rib waveguide structure was utilized in order to simplify the fabrication process. In this configuration grooves are formed on the under cladding layer by UV-imprinting and core material is spin coated on top to fill the grooves. Glucose water solution was used to characterize the sensor response against ambient refractive index changes. The sensing responses correspond linearly with the refractive index change of glucose solutions with a detection limit of about 10-5. To verify the capability of the polymer sensor for biosensing, an immunoassay was performed with c-reactive protein (CRP) and human CRP specific antibody adsorbed on the waveguide surface as a receptor. The CRP solution in PBS (phosphate buffered saline) buffer with a concentration of 2 µg/ml (16 nM) resulted in an obvious response which was over a couple hundred times of the noise level. Based on these values, a detection limit of about 2.4 pg/mm2 was found for the surface sensing of molecular adsorption. With the proposed waveguide configuration, the fabrication of polymer sensors can be ultimately transferred to roll-to-roll mass production to produce low-cost disposable sensors.

© The Authors. All rights reserved. [DOI: 10.2971/jeos.2012.12019]

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References


H.-M. Haake, A. Schtz, and G. Gauglitz, "Label free detection of biomolecular interaction by optical sensor," Fresen. J. Anal. Chem. 366, 576-585(2000).

H. Mukundan, A. Anderson, W. Grace, K. Grace, N. Hartman, J. Martinez, and B. Swanson, "Waveguide-Based Biosensors for Pathogen Detection," Sensors 9, 5783-5809 (2009).

R. D. Harris, and J. S. Wilkinson, "Waveguide surface plasmon resonance sensors," Sensor. Actuat. B-Chem. 29, 261-267 (1995).

J. Dostlek, J. Ctyroky, J. Homola, E. Brynda, M. Skalsky, P. Nekvindov, J. ?Spirkov, et al., "Surface plasmon resonance biosensor based on integrated optical waveguide," Sensor. Actuat. B-Chem. 76, 8-12 (2001).

A. Ksendzov and Y. Lin, "Integrated optics ring-resonator sensors for protein detection," Opt. Lett. 30, 3344-3346 (2005).

A. Yalin, K. C. Popat, J. C. Aldridge, T. A. Desai, J. Hryniewicz, N. Chbouki, B. E. Little, et al., "Optical sensing of biomolecules using microring resonators," IEEE J. Sel. Top. Quant. 12, 148-154 (2006).

C. Barrios, K. Gylfason, B. Snchez, A. Griol, H. Sohlstrm, M. Holgado, and R. Casquel, "Slot-waveguide biochemical sensor," Opt. Lett. 32, 3080-3082 (2007).

W. Lukosz, D. Clerc, M. Ph. Nellen, Ch. Stamm, P. Weiss, "Output grating couplers on planar optical waveguides as direct immunosensors," Biosens. Bioelectron. 6, 227-232. (1991).

N. Kim, I.-S. Park, W.-Y. Kim, "Salmonella detection with a direct- binding optical grating coupler immunosensor," Sensor. Actuat. BChem. 121, 606-615 (2007)

F. Prieto, B. Seplveda, A. Calle, A. Llobera, C. Domnguez, and L. M Lechuga, "Integrated Mach-Zehnder interferometer based on ARROW structures for biosensor applications," Sensor. Actuat. BChem. 92, 151-158 (2003)

A. Ymeti, J. S. Kanger, J. Greve, G. A. J. Besselink, P. V. Lambeck, R. Wijn, and R. G. Heideman, "Integration of microfluidics with a four-channel integrated optical Young interferometer immunosensor," Biosens. Bioelectron. 20, 1417-1421 (2005)

J. Xu, D. Suarez, and D. S. Gottfried, "Detection of avian influenza virus using an interferometric biosensor," Anal. Bioanal. Chem. 389, 1193-1199 (2007).

T. Zhang, P. Pathak, S. Karandikar, R. Giorno, and L. Que, "A polymer nanostructured Fabry-Perot interferometer based biosensor," Biosens. Bioelectron. 30, 128-132 (2011).

F. S. Ligler, and C. R. Taitt, Optical Biosensors, Second Edition: Today and Tomorrow (Elsevier Science, Mnchen, 2008).

W. Lukosz, "Principles and sensitivities of integrated optical and surface plasmon sensors for direct affinity sensing and immunosensing," Biosens. Bioelectron. 6, 215-225 (1991).

G. H. Cross, Y. Ren, and N. J. Freeman, "Young's fringes from vertically integrated slab waveguides: Applications to humidity sensing," J. Appl. Phys. 86, 6483 (1999).

R. G. Hunsperger, Integrated optics theory and technology (Springer, Berlin, 2009).

L. J. Guo, "Nanoimprint Lithography: Methods and Material Requirements," Adv. Mater. 19, 1521-4095 (2007).

J. Hiltunen, M. Hiltunen, J. Puustinen, J. Lappalainen, and P. Karioja, "Fabrication of optical waveguides by imprinting: Usage of positive tone resist as a mould for UV-curable polymer," Opt. Express 17, 22813-22822 (2009).

M. Wang, J. Hiltunen, S. Uusitalo, J. Puustinen, J. Lappalainen, P. Karioja, and R. Myllyl, "Fabrication of optical inverted-rib waveguides using UV-imprinting," Microelectron. Eng. 88, 175-178 (2011).

U. Streppel, P. Dannberg, C. Wchter, A. Bruer, L. Frhlich, R. Houbertz, and M. Popall, "New wafer-scale fabrication method for stacked optical waveguide interconnects and 3D micro-optic structures using photoresponsive (inorganic-organic hybrid) polymers," Opt. Mater. 21, 475-483 (2003).

K. Tiefenthaler, and W. Lukosz, "Sensitivity of grating couplers as integrated-optical chemical sensors," J. Opt. Soc. Am. B 6, 209-220 (1989).

M. B. Pepys, and G. M. Hirschfield, "C-reactive protein: a critical update," J. Clin. Invest. 111, 1805-1812 (2003).