Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani; L. Ciaccheri; L. Giannelli; A. A. Mencaglia

doi:10.2971/jeos.2012.12003

Journal of the European Optical Society - Rapid publications, Vol 7 (2012)

Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani, L. Ciaccheri, L. Giannelli, A. A. Mencaglia

Abstract

An experimental study was carried out, aimed at optimizing the optical/geometrical configuration for measuring the concentration of biological cells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented, as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered as test samples of the most popular analyses – cervical cells and urine, respectively. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated.

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