Single scattering detection in turbid media using single-phase structured illumination filtering

E. Berrocal; J. Johnsson; E. Kristensson; M. Alden

doi:10.2971/jeos.2012.12015

Journal of the European Optical Society - Rapid publications, Vol 7 (2012)

Single scattering detection in turbid media using single-phase structured illumination filtering

E. Berrocal, J. Johnsson, E. Kristensson, M. Alden

Abstract

This work shows a unique possibility of visualizing the exponential intensity decay due to light extinction, when laser adiation propagates through a homogeneous scattering edium. This observation implies that the extracted intensity mostly riginates from single scattering events. The filtering of this single light scattering intensity is performed by means of a single-phase structured illumination filtering approach. Results from numerical Monte Carlo simulation confirm the experimental findings for an extinction coefficient of μ_e = 0.36 mm^-1. This article demonstrates an original and reliable way of measuring the extinction coefficient of particulate turbid media based on sidescattering imaging. Such an approach has capabilities to replace the commonly used transmission measurement within the intermediate single-to multiple scattering regime where the optical depth ranges between 1 < OD < 10. The originality of the presented approach is that only one image is used (instead of three images usually employed in structured illumination) and that no monitoring of the incident intensity is required, simplifying the experimental procedure and set-up. Applications of the technique has potential in probing challenging homogeneous scattering media, such as biomedical tissues, turbid emulsions, etc, in situations where dilution cannot be applied and where conventional transmission measurements fail.

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References

V. V. Tuchin, Handbook of optical biomedical diagnostics (SPIE Press, Bellingham, 2002).

E. Berrocal, Multiple scattering of light in optical diagnostics of dense sprays and other complex turbid media, (Ph.D. Thesis, Cranfield University, England, 2006).

L. Wang, X. Liang, P. Galland, P. P. Ho, and R. R. Alfano, "True scattering coefficients of turbid matter measured by early-time gating," Opt. Lett. 20, 913-915 (1995).

A. Ansmann, M. Riebesell, and C. Weitkamp, "Measurement of atmospheric aerosol extinction profiles with a Raman lidar," Opt. Lett. 15, 746-748 (1990).

E. Berrocal, D. Y. Churmakov, V. P. Romanov, M. C. Jermy, and I. V. Meglinski, "Crossed source-detector geometry for a novel spray diagnostic: Monte Carlo simulation and analytical results," Appl. Optics 44, 2519-2529 (2005).

E. Berrocal, E. Kristensson, M. Richter, M. Linne, and M. Aldén, "Application of structured illumination for multiple scattering suppression in planar laser imaging of dense sprays," Opt. Express 16, 17870-17881 (2008).

E. Kristensson, E. Berrocal, M. Richter, S.-G. Pettersson, and M. Aldén, "High-speed structured planar laser illumination for contrast improvement of two-phase flow images," Opt. Lett. 33, 2752-2754 (2008).

T. Breuninger, K. Greger, and E. H. Stelzer, "Lateral modulation boosts image quality in single plane illumination fluorescence microscopy," Opt. Lett. 32, 1938-1940 (2007).

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, "Fast high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy," Nat. Methods 7, 637-642 (2010).

E. Kristensson, E. Berrocal, M. Richter, and M. Aldén, "Nanosecond structured laser illumination planar imaging for single-shot imaging of dense sprays," Atomization and Sprays 20, 337-343 (2010).

E. Kristensson, L. Araneo, E. Berrocal, J. Manin, M. Richter, M. Aldén, and M. Linne, "Analysis of multiple scattering suppression using structured laser illumination planar imaging in scattering and fluorescing media," Opt. Express 19, 13647-13663 (2011).

E. Kristensson, E. Berrocal and M. Aldén, "Extinction coefficient imaging of turbid media using dual structured laser illumination planar imaging," Opt. Lett. 36, 1656-1658 (2011).

L. Wang, S. L. Jacques, L. Zheng, "MCML - Monte Carlo modeling of light transport in multi-layered tissues," Comput. Methods Programs Biomed. 47, 131-146 (1995).

C. Macdonald and I. V. Meglinski, "Backscattering of circular polarized light from a disperse random medium influenced by optical clearing," Laser Phys. Lett. 8, 324-328 (2011).

E. Berrocal, D. L. Sedarsky, M. E. Paciaroni, I. V. Meglinski, and M. A. Linne, "Laser light scattering in turbid media Part I: Experimental and simulated results for the spatial intensity distribution," Opt. Express 15, 10649-10665 (2007).

X. Ma, J. Lu, S. Brocks, K. Jacob, P. Yang and X.-H. Xin, "Determination of complex refractive index of polystyrene microspheres from 370 to 1610 nm," Phys. Med. Biol. 48, 4165-4172 (2003).

C. Bohren, and D. Huffman, Absorption and scattering of light by small particles (Wiley, New York, 1983).

H. C. van de Hulst, Light scattering by small particles (Dover, New York, 1981).