Journal of the European Optical Society - Rapid publications, Vol 4 (2009)

Multi-particle three-dimensional coordinate estimation in real-time optical manipulation

J. S. Dam, I. Perch-Nielsen, D. Palima, J. Glückstad


We have previously shown how stereoscopic images can be obtained in our three-dimensional optical micromanipulation system [J. S. Dam et al, Opt. Express 16, 7244 (2008)]. Here, we present an extension and application of this principle to automatically gather the three-dimensional coordinates for all trapped particles with high tracking range and high reliability without requiring user calibration. Through deconvolving of the red, green, and blue colour planes to correct for bleeding between colour planes, we show that we can extend the system to also utilize green illumination, in addition to the blue and red. Applying the green colour as on-axis illumination yields redundant information for enhanced error correction, which is used to verify the gathered data, resulting in reliable coordinates as well as producing visually attractive images.

© The Authors. All rights reserved. [DOI: 10.2971/jeos.2009.09045]

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D. G. Grier, "A revolution in optical manipulation" Nature 424, 810- 816 (2003).

A. Ashkin, "Acceleration and trapping of particles by radiation pressure" Phys. Rev. Lett. 24, 156-159 (1970).

P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Real-time threedimensional optical micromanipulation of multiple particles and living cells" Opt. Lett. 29, 2270-2272 (2004).

A. Isomura, N. Magome, M. I. Kohira, K. Yoshikawa, "Toward the stable optical trapping of a droplet with counter laser beams under microgravity" Chem. Phys. Lett. 429, 321-325 (2006).

G. M. Whitesides, "The origins and the future of microfluidics" Nature 442, 368-373 (2006).

J. S. Dam, I. R. Perch-Nielsen, D. Palima, and J. Glückstad, "Threedimensional imaging in three-dimensional optical multi-beam micromanipulation" Opt. Express 16, 7244-7250 (2008).

S. H. Lee, and D. G. Grier, "Holographic microscopy of holographically trapped three-dimensional structures" Opt. Express 15, 1505- 1512 (2007).

I. Perch-Nielsen, P. Rodrigo, and J. Glückstad, "Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes" Opt. Express 13, 2852-2857 (2005).

P. J. Rodrigo, L. Kelemen, C. A. Alonzo, I. R. Perch-Nielsen, J. S. Dam, P. Ormos, and J. Glückstad, "2D optical manipulation and assembly of shape-complementary planar microstructures" Opt. Express 15, 9009-9014 (2007) . [10] J. Konrad, B. Lacotte, and E. Dubois, "Cancellation of image crosstalk in time-sequential displays of stereoscopic video" IEEE T. Image Process. 9, 897-908 (2000).

M. Cheezum, W. Walker, and W. Guilford, "Quantitative comparison of Aalgorithms for tracking single fluorescent particles" Biophys. J. 81, 2378-2388 (2001).